Description:
This product is obtained by deep fermentation of Bacillus licheniformis, and it is widely used in the manufacturing of starch sugar, beer saccharification, organic acid (salt), monosodium glutamate and alcohol production.
Technical information:
- Available in liquid (brown) 1,000 and 2,000 u/ml.
- Temperature range: 40-65℃ (Optimum temperature: 60—65℃).
- pH range: 4.0-7.0 (Optimum pH: 4.2-4.8)
- Unit definition: The amount of enzyme needed to liberate 1 mg of reducing sugar (calculated as glucose) from pullulan in one minute. (Standard: LDZJ 03.02-2009)
Mode of Action:
Pullulanase is a debranching isoamylase that hydrolyzes pululan, amylopectin and α-1.6 glucosidic linkages and during saccharification converts them in fermentable sugars. Works very well in conjunction with glucoamylase and fungal-α-amylase.
Uses:
- Crystalline dextrose
In the manufacturing of crystalline dextrose or organic acid (salt) with glucose as a substrate, glucoamylase will continue the saccharification of partially processed starch and dextrin into glucose. If the maltose content exceeds 55%, the use of Pullulanase and β-amylase will be required.
Recommended dosage:
- Beer
In beer manufacturing, Pullulanase will reduce the content of amylopectin to produce a dry beer. It also reduces the use of Glucoamylase and shortens the fermentation process by reducing the saccharification time.
Recommended dosage: 10-50 ml/ton of wort, based on 1,000 u/ml.
- Modified starch
In the manufacturing of modified starch, Pullulanase can increase the amylose content transforming the film-forming ability and solubility of anti-peptic products.
Recommended dosage: 0.6 to 2.0 L/ton of raw material based on 1,000 u/ml as initial dose for a 6 to 12 hr.
- Other applications
Pullulanase can be used in the manufacturing of monosodium glutamate, alcohol, vinegar, soy sauce, and yellow rice wine during the saccharification phase reducing residual dextrins and improving the use of the substrate.
Packaging, Storage & Handling
- Liquid: Plastic drums of 22; 200; 840 and 1,000 L.
- Powder: 20 kg cartons; 25 kg bags and 25 kg drums.
- Storage & handling: Avoid exposure to direct sunlight; keep in clean, cool, and dry place.