Cellulase
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Cellulase
Description:
Cellulase is obtained through submerged fermentation of Trichoderma reesei, it is available in liquid and powder and has the ability to degrade cellulose into cello-oligosaccharide, cellobiose and glucose. It is widely used in the feed, distilling and textile industries.
Technical information:
- Available in liquid (brown) 100,000-200,000 u/ml and powder form (off white) 40,000; 100,000; 200,000 u/g. Food grade 400,000 u/g.
- Temperatura range: 30 to 75℃ (Optimum temperature: 55 to 60℃).
- pH range: 4.3-6.0 (Optimum pH: 4.5-5.0)
- Unit definition: (CMCA ENZYME): The amount of enzyme needed to hydrolyze cellulose in one hour at a pH 4.8 at 50℃, producing the equivalent to 1 mg of glucose (Standard: QB 2583-2003)
Mode of Action:
Cellulase is a linear polysaccharide of glucose residues connected by β-1.4 linkages. Cellulases are a multicomponent enzymatic system with endoglucanases (EG) that randomly hydrolyze cellulase chains into cellobiohydrolases (CBH) that hydrolyze cellobiose from the polymer ends and cellobiases that hydrolyze cellobiose into glucose and β-glucanases.
Uses:
- Feed
Because of its cellulolytic properties, Cellulase improves the digestibility and utilization of fibrous nutrients, and it is commonly used in monogastric and ruminant diets.
Recommended dosage: 0.03-0.1 kg/ton of raw material based on 100,000 u/ml.
- Textiles
In textiles, Cellulase is used for stonewashing by reducing the tear strength and dye uptake and to soften the texture of fabrics.
Recommended dosage: 0.5-2.0 (owf) at pH 4.5-5.5 at 45-55℃. Bath ratio: 1:10-25 for 30 to 60 minutes.
- Alcohol
Because of its high starch content, cassava is an attractive feedstock for ethanol production. The use of Cellulase, in solid fermentation, will enhance alcohol production by converting unused glucan components into glucose and then into ethanol. A similar effect occurs when Cellulase is used in the elaboration of rice wine.
Recommended dosage: 0.15-0.25 kg/ton of raw material.
- Juice production
Cellulase helps to eliminate haziness in apple juice by hydrolyzing the cellulose naturally present in apples.
Recommended dosage: 0.05-0.4 L/ton of pulp based on 100,000 u/ml for 1 to 5 hrs. at a of pH 4.5-5.5, and at 45-60℃
Packaging, Storage & Handling
- Liquid: Plastic drums of 22; 200; 840 and 1,000 L.
- Powder: 20 kg cartons; 25 kg bags and 25 kg drums.
- Storage & handling: Due to its organic nature, high temperature, strong acid & strong alkaline conditions will inactivate the enzyme.
- Avoid exposure to sunlight, keep in clean, cool, and dry place