Description:
β-Amylase is a high efficiency enzyme obtained from plants through Lonct Enzyme proprietary methods and characterized by good tolerance to high temperatures. This enzyme acts on starch, glycogen and related polysaccaharides and oligosaccharides producing β-maltose by an inversion. β-Amylase cleaves two bound glucose molecules (maltose) from the reducing end of the chain. It is widely used in the manufacturing of beer, vinegar, high maltose syrup, and crystalline maltitol.
Technical information:
- Available in liquid (dark brown) 700,000 u/ml.
- Temperature range: Stable below 65℃ (Optimum temperature: 55-60℃).
- pH range: 3.0-6.5 (Optimum pH: 5.0-5.6)
Unit definition: The amount enzyme required by hydrolysis of 1.10% starch solution to produce 1 mg of maltose per hour at pH 5.5 and 60℃. (Standard: LDZJ 03 04-2010)
Mode of Action:
β amylase is an exoamylase enzyme, which starts from the non-reductive end of liquefied starch and hydrolyzes α-1.4-glucosidic linkages in polysaccharides resulting in the successive liberation of maltose units from the non-reducing ends of the chain and simultaneously undergoing a Walden Inversion Reaction by transforming α-maltose into β-maltose.
The optimum saccharification conditions depend on saccharification time, pH, temperature, substrate concentration, and enzyme dosage. So, it is up to the plant to decide which are the optimum conditions for each particular case.
Uses:
- Maltose syrup and beer brewing
Recommended dosage: 0.1 to 0.3 L/ton of dry material.
Optimum saccharification time for maltose syrup: 12 to 30 hours.
Optimum saccharification time for beer brewing: 0.5 to 1 hour.
- High maltose syrup
For the production of high maltose syrup, (>70%) The saccharification stage will require the addition of Pullulanase.
Recommended dosage: β-Amylase 0.5 to 1.0 L/ton of dry material and Pullulanase 0.5 to 1.0 L/ton of dry material based on 1,000 u/ml. Saccharification time: 24 hrs.
- Super high maltose syrup
For the production of super high maltose syrup, (>90%) The saccharification stage will require the addition of Pullulanase.
Recommended dosage: β-Amylase 1.5 to 2.0 L/ton of dry material and Pullulanase 1.5 to 2.8 L/ton of dry material based on 1,000 u/ml. Saccharification time: 24 hrs. Adding maltogenic amylase 0.0.15 L/ton of dry basis (enzyme activity based on 2000 u/ml).
Packaging, Storage & Handling
- Liquid: Plastic drums of 22; 200; 840 and 1,000 L.
- Powder: 20 kg cartons; 25 kg bags and 25 kg drums.
- Storage & handling: Avoid exposure to direct sunlight; keep in clean, cool, and dry place.